Journal: bioRxiv

Article Title: Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery

doi: 10.1101/599514

Figure Lengend Snippet: (a) A heatmap showing expression of 37 significantly upregulated genes in cancer liquid biopsies. (b,c) Among 37 upregulated genes, 13 genes ( FH, SMARCB1, GRHPR, PHB, NACA, RPS11, RPL22, NPM1, EPCAM, TFG, HDAC6, ELK4, BRD3 ) were significantly upregulated in primary tumors (n=497) compared to normal (n=52) in TCGA data. In the heatmap, black dots next to the gene name mark the genes upregulated in primary tumor compare to normal in TCGA data. The TCGA project for PCa data is publicly available for download at https://portal.gdc.cancer.gov/projects/TCGA-PRAD . (d) These 13 genes were also tested in two prostate cancer cell lines (LNCaP and PC3), and most were overexpressed apart from NACA , which was downregulated in both cell lines; BRD3 and EPCAM , which were decreased in PC3 cells, and HDAC6 , which was downregulated in LNCaP cells. (e) Normalized Enrichment Score plot of the top 13 pathways in cancer urine. (f) Geneset Enrichment Score Plot (GSEA) of the TCA cycle (NES = 2.8, p-value = 0.0). (g) GSEA of the alanine, aspartate, and glutamate metabolism (NES = 2.42, p-value = 0.0). GSEA was conducted using GSEA software from the Broad Institute ( http://software.broadinstitute.org/gsea/index.jsp ).

Article Snippet: Prostate cancer cell lines LNCaP (ATCC ® CRL-1740TM) and PC3 (ATCC ® CRL-7934TM) were cultured in RPMI 1640 medium and Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific), respectively, supplemented with 10% FBS and penicillin/streptomycin.

Techniques: Expressing, Software

Journal: bioRxiv

Article Title: Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery

doi: 10.1101/599514

Figure Lengend Snippet: (a) GOT1 knockdown in LNCaP and PC3 prostate cancer cell lines. (b) GOT1 knockdown significantly inhibits cell viability in PC3 and LNCaP cells. (c) Invasion and (d) anchorage-independent growth in prostate cancer cell lines upon GOT1 knockdown. (e) GOT1 knockdown significantly increases ROS production in PC3 and LNCaP cells. The data from three independent experiments were expressed as mean ± SD.

Article Snippet: Prostate cancer cell lines LNCaP (ATCC ® CRL-1740TM) and PC3 (ATCC ® CRL-7934TM) were cultured in RPMI 1640 medium and Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific), respectively, supplemented with 10% FBS and penicillin/streptomycin.

Techniques: Knockdown

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Corosolic acid, a natural triterpenoid, induces ER stress-dependent apoptosis in human castration resistant prostate cancer cells via activation of IRE-1/JNK, PERK/CHOP and TRIB3

doi: 10.1186/s13046-018-0889-x

Figure Lengend Snippet: CA suppresses cell proliferation and induces apoptosis in human prostate cancer cells. a Structure of the CA molecule. b PC-3, DU145, 22rv1 and WPMY-1 cells were treated with various concentrations of CA for 24 and 48 h, cell viability was assayed by MTT assay. c Apoptosis index in PC-3 and DU145 cells with 0, 5, 10 and 15 μM CA treatments for 24 h detected by annexinV/PI flow cytometry assay. d Statistical analysis result of flow cytometric analysis of apoptosis (both of early and later apoptosis). e Cell proliferation was measured by colony formation in 12-well plates with crystal violet staining. Representative photographs are shown. f The percentage of colony formation was calculated by defining the number of colonies in the absence of CA as 100%. The results are presented as mean ± SD and described as column chart * p < 0.05 and ** p < 0.01 as compared with untreated control

Article Snippet: The human prostate cancer cell line 22RV1, PC-3 and DU145 as well as normal prostate cells (WPMY-1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: MTT Assay, Flow Cytometry, Staining, Control