Journal: bioRxiv

Article Title: Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery

doi: 10.1101/599514

Figure Lengend Snippet: (a) A heatmap showing expression of 37 significantly upregulated genes in cancer liquid biopsies. (b,c) Among 37 upregulated genes, 13 genes ( FH, SMARCB1, GRHPR, PHB, NACA, RPS11, RPL22, NPM1, EPCAM, TFG, HDAC6, ELK4, BRD3 ) were significantly upregulated in primary tumors (n=497) compared to normal (n=52) in TCGA data. In the heatmap, black dots next to the gene name mark the genes upregulated in primary tumor compare to normal in TCGA data. The TCGA project for PCa data is publicly available for download at https://portal.gdc.cancer.gov/projects/TCGA-PRAD . (d) These 13 genes were also tested in two prostate cancer cell lines (LNCaP and PC3), and most were overexpressed apart from NACA , which was downregulated in both cell lines; BRD3 and EPCAM , which were decreased in PC3 cells, and HDAC6 , which was downregulated in LNCaP cells. (e) Normalized Enrichment Score plot of the top 13 pathways in cancer urine. (f) Geneset Enrichment Score Plot (GSEA) of the TCA cycle (NES = 2.8, p-value = 0.0). (g) GSEA of the alanine, aspartate, and glutamate metabolism (NES = 2.42, p-value = 0.0). GSEA was conducted using GSEA software from the Broad Institute ( http://software.broadinstitute.org/gsea/index.jsp ).

Article Snippet: Prostate cancer cell lines LNCaP (ATCC ® CRL-1740TM) and PC3 (ATCC ® CRL-7934TM) were cultured in RPMI 1640 medium and Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific), respectively, supplemented with 10% FBS and penicillin/streptomycin.

Techniques: Expressing, Software

Journal: bioRxiv

Article Title: Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery

doi: 10.1101/599514

Figure Lengend Snippet: (a) GOT1 knockdown in LNCaP and PC3 prostate cancer cell lines. (b) GOT1 knockdown significantly inhibits cell viability in PC3 and LNCaP cells. (c) Invasion and (d) anchorage-independent growth in prostate cancer cell lines upon GOT1 knockdown. (e) GOT1 knockdown significantly increases ROS production in PC3 and LNCaP cells. The data from three independent experiments were expressed as mean ± SD.

Article Snippet: Prostate cancer cell lines LNCaP (ATCC ® CRL-1740TM) and PC3 (ATCC ® CRL-7934TM) were cultured in RPMI 1640 medium and Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific), respectively, supplemented with 10% FBS and penicillin/streptomycin.

Techniques: Knockdown

Journal: Contrast Media & Molecular Imaging

Article Title: High Contrast PET Imaging of GRPR Expression in Prostate Cancer Using Cobalt-Labeled Bombesin Antagonist RM26

doi: 10.1155/2017/6873684

Figure Lengend Snippet: (a) In vitro specificity test of 57 Co-NOTA-PEG 2 -RM26 binding to GRPR on PC-3 (human prostate cancer) cells. Presaturation of receptors with unlabeled NOTA-PEG 2 -RM26 caused significant ( p < 0.05) reduction of cell-bound 57 Co-NOTA-PEG 2 -RM26 radioactivity. (b) Binding and internalization of 57 Co-NOTA-PEG 2 -RM26 at 37°C by PC-3 cells. Data are normalized to a maximum cell-bound radioactivity and presented as average value from 3 cell dishes ± SD. Not all error bars are visible due to the small standard deviations.

Article Snippet: PC-3 human prostate cancer cells expressing GRPR were purchased form ATCC via LGC Promochem.

Techniques: In Vitro, Binding Assay, Radioactivity